Phenotypic features and analysis of genes supporting probiotic action unravel underlying perspectives of Bacillus velezensis VTX9 as a potential feed additive for swine

Abstract Purpose To date, a total of 13 probiotic Bacillus species are considered as Generally Recognized Safe organism (GRAS) approved by the US Federal Food, Drug, and Cosmetic Act (FDCA), which used for food feed additives. However, velezensis is not candidate in swine farming due to lack genetic basis action-related traits. Therefore, present study was undertaken exploit underlying traits B. VTX9. Methods The genome sequencing VTX9 performed on PacBio Sequel platform. properties including biosafety, antioxidative capacity, riboflavin exopolysaccharide production were evaluated using genotypic phenotypic analysis. secondary metabolite potentials also predicted. Results Strain isolated from feces proved some resistance 3 mM H 2 O , 0.6 bile salt, low pH, antipathogenic activity. complete consists 3,985,800 bp chromosome that housed 3736 protein-coding genes 5 plasmids with size ranging 7261 20,007 bp. Genome analysis revealed no functional encoding enterotoxins transferable antibiotic resistance, confirmed safety A 82 involved gastrointestinal stress tolerance predicted, has been reported previously. maximum reached 769 ± 7.5 ng/ml LB medium after 72 h, agreement de novo biosynthetic pathway exploited first time genome. Antagonistic activity against pathogenic bacteria attributed 10 metabolites clusters. presence large gene cluster biosynthesis exopolysaccharides underscored further adhesion biofilm-forming capabilities intestines. Conclusion Our results potential be candidate. information provided here opens new opportunities additive future.